• Inverted optical microscope for in vivo cell culture observation with camera for imagine acquisition
  Visiscope 400 series and camera for imagine acquisition (movie and photo) connected to a laptop
• Microbiological safety cabinet – Class II
  MN120 Nüve Class II microbiological safety cabinet, with UV lamp (220 V, 50 H) sterilization system
• Unstirred water bath
  Nüve bath NB9, 7 L capacity
• CO2 incubator for human cell culture maintenance
  New Brunswick™ Galaxy® 170 S CO2 Incubator (Eppendorf), 170 L capacity
• Peltier-cooled incubator for insect cell culture maintenance
  Memmert IPP260, 256 L capacity, setting temperature range 0-70°C, double door
• GERMREDUC continuous airflow air cleaner
  Wall unit, UV rays (λ =254 nm), viable with employee attendance, 92% air purification degree, 220 V, 50 Hz, lamp life: 6,000 hours, one aspiration module with 2 UV lamps, 128 m3/hr airflow rate
• Stereo microscope
  Greenough Leica 6.3:1 zoom ratio, with a magnification range of 6.3X - 80X, configuration for reflected light, integrated video/photo port
• Refrigerator/freezer
  Electrolux
• Mitsubishi air conditioning
  Monosplit inverter system with heat pump according to ErP regulation. R410A ecological gas for condition, heat, dehumidify and purify the air.

The laboratory deals with:

• Cultivation of human intestinal cells of tumour and non-tumour origin
• Construction of three-dimensional human intestinal models with different types of epithelial cells or PBMC
• Microorganisms and human intestinal models interaction assays
• Immunological (ELISA), molecular (RT-qPCR, RNA-seq), proliferative and physiological assays applied to human cells in co-culture
• Host/human intestinal model interaction analysis through Tras-epithelial Electrical Resilience (TEER)
• Primary cultures on insect immunocytes
• Insect immune system and insect-symbiotic and non-symbiotic microorganisms interaction analysis
• Advice in experimental design (human/microorganism and insect/microorganism studying models)
• New users training
• Technical assistance in data interpretation.

The laboratory is accessible only on request accompanied by authorised staff.
Fees and access methods are defined according to specific requests.